Cationic polymer and lipoprotein-containing system for gene delivery
AssigneeUniversity of Utah Research Foundation
InventorsJin-Seok Kim, Atsushi Maruyama, Toshihiro Akaike, Sung Wan Kim
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Abstract
A composition for delivery of a selected nucleic acid into a targeted host cell comprises a complex of a hydrophobized, positively charged, biocompatible polymer; a lipoprotein; and a selected nucleic acid. Hydrophobic and electrostatic interactions between the components provide for the formation of the condensed DNA-containing complex. Preferred embodiments of the complex have a slightly positive surface charge and an average diameter of about 200 nm. Plasmids and oligonucleotides can be efficiently delivered with such compositions. A method of delivering a selected nucleic acid to a host cell is also disclosed.
Claims32
A composition for delivery of a selected nucleic acid into a targeted host cell comprising a complex comprising an effective amount of a hydrophobized, positively charged, biocompatible polymer;
- an effective amount of a lipoprotein;
- and an effective amount of said selected nucleic acid.
A method of transforming a cell in vitro with a selected nucleic acid comprising the steps of:
- (a) providing a composition comprising a complex comprising an effective amount of a hydrophobized, positively charged, biocompatible polymer;
- an effective amount of a lipoprotein;
- and an effective amount of said selected nucleic acid;
- (b) contacting said cell with an effective amount of said composition such that said cell internalizes said selected nucleic acid;
- and
- (c) culturing said cell with said internalized selected nucleic acid under conditions favorable for growth of said cell.
Dependencies are inferred from the claim text.