# US 5,679,551 A · Unique double-stranded RNAS associated with the Trichomonas vaginalis virus

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- Number: US 5,679,551 A
- Document ID: US5679551A
- Status: Granted
- Kind: A
- Application number: 08/551,275
- Filed: 1995-10-31
- Granted: 1997-10-21
- Earliest claimed: 1995-10-31
- Assignees: Board of Regents, The University of Texas System
- Inventors: John F. Alderete
- Web page: https://patentgrep.com/patent/US5679551A

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C07K 14/005: from viruses
- C12N 15/79: Vectors or expression systems specially adapted for eukaryotic hosts
- C12N 2720/00022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2720/00051: Methods of production or purification of viral material
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Disclosed are nucleic acid sequences encoding satellite RNA's associated with the dsRNA virus of Trichomonas vaginalis. The use of the satellite molecules as vectors for the insertion of foreign genetic material into Trichomonas vaginalis protozoans infected with dsRNA virus is also disclosed.

## Claims

### Claim 1

An isolated nucleic acid comprising a contiguous sequence consisting of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 2 (depends on 1)

The isolated nucleic acid of claim 1, wherein said consists of the sequence of SEQ ID NO:1 or its full length complement, or SEQ ID NO:3 or its full length complement.

### Claim 3 (depends on 1)

The isolated nucleic acid of claim 1, wherein said nucleic acid segment comprises a cDNA molecule.

### Claim 4 (depends on 1)

A recombinant vector comprising the isolated nucleic acid of claim 1.

### Claim 5 (depends on 4)

The recombinant vector of claim 4, wherein said vector replicates in a TVV infected Trichomonas vaginalis host.

### Claim 6 (depends on 4)

A protozoan cell containing the recombinant vector of claim 4.

### Claim 7 (depends on 6)

The protozoan cell of claim 6, wherein said cell is a Trichomonas vaginalis cell.

### Claim 8

A method of expressing a recombinant gene in a TVV infected protozoan cell comprising the steps of:
(a) preparing a nucleic acid molecule comprising a recombinant gene fused with a sequence in accordance with SEQ ID NO:1 or SEQ ID NO:3;
(b) transfecting said molecule into a TVV infected protozoan cell; and
(c) culturing said cell under conditions appropriate for the expression of said recombinant gene.

### Claim 9 (depends on 8)

The method of claim 8 wherein said protozoan cell is a Trichomonas vaginalis cell.

### Claim 10

An isolated nucleic acid which comprises at least 40 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 11 (depends on 10)

An isolated nucleic acid of claim 10, which comprises at least 50 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 12 (depends on 11)

An isolated nucleic acid of claim 11, which comprises at least 75 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 13 (depends on 12)

An isolated nucleic acid of claim 12, which comprises at least 100 contiguous nucleotides of SEQ 12D NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 14 (depends on 13)

An isolated nucleic acid of claim 13, which comprises at least 150 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ I12) NO:1 or SEQ ID NO:3.

### Claim 15 (depends on 14)

An isolated nucleic acid of claim 14, which comprises at least 200 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 16 (depends on 15)

An isolated nucleic acid of claim 15, which comprises at least 300 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 17 (depends on 16)

An isolated nucleic acid of claim 16, which comprises at least 400 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 18 (depends on 17)

An isolated nucleic acid of claim 17, which comprises at least 500 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 19 (depends on 18)

An isolated nucleic acid of claim 18, which comprises at least 600 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 20 (depends on 19)

An isolated nucleic acid of claim 19, which comprises at least 616 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3 or the full length complement of SEQ ID NO:1 or SEQ ID NO:3.

### Claim 21 (depends on 20)

An isolated nucleic acid of claim 20, which comprises at least 688 contiguous nucleotides of SEQ ID NO:1 or its full length complement.