Protein microspheres and methods of using them

A method for adhering substances to tissue and enamel comprising administering the substances incorporated into prolamine microspheres produced by

• a) contacting a protein solution, wherein the protein consists of prolamine, and contains biologically active compound to be incorporated, with an oil, which is of limited miscibility with the prolamine solvent and does not dissolve the prolamine, to form a prolamine-oil mixture having a ratio of 1:18.75, prolamine solvent:oil;
• b) agitating the prolamine-oil mixture to form a dispersion of the prolamine solution in the oil;
• and
• c) extracting the prolamine solvent into the oil to form stable prolamine microspheres having dispersed throughout the prolamine the biologically active compound,
• wherein all of the procedures are performed at a temperature which does not denature the prolamine and the resulting prolamine microsphere has a diameter between 50 nm and 100 microns and releases incorporated compound in a controlled manner in the absence of amide linkages or heat denaturation of the prolamine.