# US 5,677,429 A · Monoclonal antibodies to the Mystery Swine Disease virus

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- Number: US 5,677,429 A
- Document ID: US5677429A
- Status: Granted
- Kind: A
- Application number: 08/488,286
- Filed: 1995-06-07
- Granted: 1997-10-14
- Earliest claimed: 1991-08-26
- Assignees: South Dakota State University
- Inventors: David A. Benfield
- Web page: https://patentgrep.com/patent/US5677429A

## CPC

- A61K 39/12: Viral antigens
- A61P 1/00: Drugs for disorders of the alimentary tract or the digestive system
- A61P 11/00: Drugs for disorders of the respiratory system
- A61P 31/04: Antibacterial agents
- A61P 31/12: Antivirals
- A61P 37/04: Immunostimulants
- C07K 16/10: RNA viruses
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 2039/552: {Veterinary vaccine}
- C12N 2720/00011: Details
- C12N 2720/00034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2770/10011: Arteriviridae
- C12N 2770/10021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2770/10034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y10S 435/975: Kit
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Monoclonal antibody sera can be produced by hybridoma technique. After immunization of a mouse, pig, rat, rabbit or other appropriate species with Mystery Swine Disease (MSD) containing cell culture lysate, the spleen of the animal can be removed and converted into a whole cell preparation. Preferably the monoclonal antibody is produced by a murine derived hybrid cell line wherein the antibody is an IgG or IgM type immunoglobulin. Example monoclonal antibodies to the infectious agent for SIRS are monoclonal antibody SDOW 12 and SDOW 17.

## Claims

### Claim 1

A monoclonal antibody produced by a murine-derived hybrid cell line wherein said antibody specifically binds to an epitope on a 15 kD antigen of viral agent ATCC VR-2332.

### Claim 2 (depends on 1)

The monoclonal antibody of claim 1 wherein said antibody is an IgG or IgM type immunoglobulin molecule.

### Claim 3 (depends on 1)

The monoclonal antibody of claim 1 wherein the antibody is produced by hybridoma cell line ATCC HB10996.

### Claim 4 (depends on 1)

The monoclonal antibody of claim 1 wherein the antibody is produced by hybridoma cell line ATCC HB10997.