Compositions and methods for phage resistance in dairy fermentations
AssigneeUtah State University, Office of Technology Commercialization
InventorsJeff R. Broadbent, Craig J. Oberg, Shelby Caldwell
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Abstract
A latococcal- and streptococcal-phage-resistant starter culture for fermenting milk comprises a food-grade bacterium from the genera Pediococcus, Leuconostoc, Lactococcus, Streptococcus, or Lactobacillus transformed with a genetic element containing genes for a lactose fermentation phenotype. A method of making a lactococcal-phage-resistant starter culture comprises transforming a non-lactose fermenting, food-grade bacterium with a genetic element carrying determinants for a lactose fermentation phenotype. A method of making cheese with lactococcal-phage-resistant starter culture is also disclosed.
Claims11
A method of making a phage resistant starter culture for fermenting milk comprising transforming a food-grade bacterium that lacks a lactose-fermenting phenotype and is resistant to lactococcal and streptococcal phages with a genetic element bearing a genetic locus for lactose fermentation to result in a transformed food-grade bacterium that is operable for replicating said genetic element, expresses a lactose fermentation phenotype, and bears no antibiotic resistance marker.
A method of making a phage-resistant starter culture for fermenting milk comprising mixing a culture of food-grade bacteria that lack a lactose-fermenting phenotype and are resistant to lactococcal and streptococcal phages with an effective amount of a genetic element bearing a genetic locus for lactose fermentation to form a mixture, wherein neither the food-grade bacteria nor the genetic element bear an antibiotic resistance marker;
- treating the mixture such that a portion of the food-grade bacteria is transformed with the genetic element to result in a mixture of transformed and untransformed bacteria;
- and selecting bacteria transformed with the genetic element from the mixture of transformed and untransformed bacteria by ability to grow on lactose as a sole carbon source.
Dependencies are inferred from the claim text.