Back to results
US 5,677,136 AGranted
Methods of obtaining compositions enriched for hematopoietic stem cells, compositions derived therefrom and methods of use thereof
AssigneeSystemix, Inc.
InventorsPaul J. Simmons, Beth L. Hill, Benjamin P. Chen
Life of this patent
Estimating the term…
Abstract
A method for obtaining human hematopoietic stem cells is provided by enrichment for stem cells using a novel stem cell marker. Compositions enriched for stem cells are obtained using novel antibody HCC-1 which is specific for a CD59 epitope highly accessible on stem cells, and less accessible or absent on mature cells. The enriched population of cells derived from these methods are also provided.
Claims26
A method of obtaining a composition substantiaily enriched in subpopulation of hematopoiefic stem cells comprising:
- (a) obtaining, a source of cells comprising human hematopoietic cells;
- (b) isolating a population of human hematopoietic cells from the source;
- (c) contacting the population of human hematopoietic cells with a monoclonal antibody specific for the marker HCC-1;
- and
- (d) separating the subpopulation that is specifically bound by the monoclonal antibody, thereby obtaining a composition substantially enriched in a subpopulation of human hematopoietic stem cells.
2 more elements
The hybridoma cell line designated ATCC Accession No. HB 11729.
Dependencies are inferred from the claim text.