Method of in vivo delivery of functioning foreign genes
AssigneeVanderbilt University
InventorKenneth L. Brigham
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Abstract
A method of expressing a foreign gene in cells of a mammalian organ in vivo includes the step of injecting genetic material capable of inducing desired gene products into a mammal. The genetic material is complexed to cationic liposome carriers. The genetic material is lipofected into cells of the mammalian organ. Gene expression and cellular function of the genetic material are activated to produce the desired foreign products in the cells of the organ.
Claims21
A method of expressing a gene predominantly in a target organ in a mammal, comprising the step of:
- administering by injection a nucleic acid liposome complex capable of functionally expressing said gene to said mammal at a site whereby said target organ is the first organ having a capillary bed proximal to the site of administration,
- wherein said nucleic acid is complexed by ionic interaction to cationic liposomes that effect delivery of said gene predominantly to said target organ and transfect said gene into a cell in said target organ, and
- wherein said gene is predominantly expressed in said target organ.
A method of expressing a gene predominantly in the lungs in a mammal comprising the step of:
- administering by inhalation a plasmid capable of functionally expressing said gene to said mammal,
- wherein said plasmid is complexed by ionic interaction to cationic liposomes that effect delivery of said gene predominantly to said lungs and transfects said gene into a cell in said lungs, and
- wherein said gene is predominantly expressed in said lungs.
A method of delivering a nucleic acid liposome complex predominantly to a target organ in a mammal, comprising the step of administering said nucleic acid liposome complex to said mammal by injection at a site whereby said target organ is the first organ having a capillary bed proximal to the site of administration,
- wherein said nucleic acid is complexed by ionic interaction to cationic liposomes that effect delivery of said nucleic acid predominantly to said target organ and transfect said nucleic acid into a cell in said target organ.
A method of delivering a nucleic acid predominantly to the lungs in a mammal, comprising the step of administering said nucleic acid by inhalation,
- wherein said nucleic acid is complexed by ionic interaction to one or more cationic liposomes that deliver said nucleic acid predominantly to said lungs and transfect said nucleic acid into a cell in said lungs.
Dependencies are inferred from the claim text.