Isolation and characterization of an actin gene from abalone
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsDennis Alpha Powers, Lynna Madsen Hereford, deceased, Marta Gomez-Chiarri
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Abstract
The present invention describes vectors and methods useful for the production of transgenic mollusks, in particular, transgenic abalone. The invention further describes transgenic mollusks having enhanced growth properties. In addition, the isolation and characterization of an abalone actin gene promoter region is disclosed.
Claims24
A transgenic abalone containing a DNA sequence heterologous to said abalone, wherein
- (i) said DNA sequence is flanked by regulatory sequences causing expression in said abalone of said DNA sequence, and
- (ii) said DNA sequence expresses a polypeptide that promotes growth enhancement of the transgenic abalone relative to wild-type abalone.
A transgenic abalone containing a DNA sequence heterologous to said abalone, wherein
- (i) said DNA sequence is flanked by regulatory sequences causing expression in said abalone of said DNA sequence, and
- (ii) said DNA sequence expresses a marker polypeptide.
An expression vector useful for transformation of an abalone comprising
- a first DNA sequence flanked by regulatory elements effective to allow expression of the sequence in said abalone, wherein said regulatory elements are derived from an abalone actin gone, and
- second DNA sequences allowing the propagation of the vector in a secondary host,
- wherein said regulatory elements include a promoter sequence derived from SEQ ID NO:5.
A method for transfecting an abalone, comprising,
- introducing an expression vector by electroporation into a fertilized abalone egg, said vector containing a DNA sequence flanked by regulatory elements effective to allow expression of the sequence in abalone,
- culturing said abalone egg under conditions that allow development of the egg into an abalone, and
- identifying a transfected abalone based on the presence of said DNA sequence.
A method for enhancing the weight of an abalone relative to a wild-type abalone, comprising
- introducing a vector by electroporation into a fertilized abalone egg, said vector containing a DNA sequence flanked by regulatory elements effective to allow expression of the sequence in abalone, where said DNA sequence encodes a gene product that promotes growth enhancement and said DNA sequence and regulatory elements become integrated into the abalone egg genome,
- culturing said abalone egg under conditions that allow development of the egg into a transgenic abalone, and
- growing said transgenic abalone under conditions causing expression of the gene product,
- wherein the weight of the transgenic abalone is enhanced relative to the weight of a wild-type abalone.
A duplex DNA fragment containing
- a DNA sequence encoding a polypeptide, adjacent said DNA sequence a promoter effective to promote transcription of said DNA sequence, where said promoter is an abalone actin gene promoter derived from SEQ ID NO:5 and said DNA sequence is heterologous to the abalone actin gene promoter.
A method for expressing a protein in an abalone, comprising
- introducing an expression vector by electroporation into a fertilized abalone egg, said vector containing a DNA sequence flanked by regulatory elements effective to allow expression of the sequence in abalone and said DNA sequence and regulatory elements becoming integrated into the abalone egg genome,
- culturing said abalone egg under conditions that allow development of the egg into an abalone, and
- growing the abalone under conditions permissive for the expression of the protein,
- wherein said growing results in expression of the protein.
Dependencies are inferred from the claim text.