Purification of cinnamoyl-C-glyoside chromone
AssigneeUnivera Phytoceuticals, Inc.
InventorsTodd Waller, Qi Jia, Abeysinghe Padmapriya
Life of this patent
Estimating the term…
Abstract
The present invention provides a process for purifying the cinnamoyl-C-glycoside, 8-C-.beta.-D-2-O-(E)-cinnamoyl!glycopyranosyl-2- (R)-2-hydroxy!propyl-7-methoxy-5-methyl-chromone, referred to herein as the "540 compound." In one embodiment of the present invention the 540 compound is purified by extraction from a decolorizing agent with an organic solvent. The extracted product can be further purified by high pressure liquid chromatography. In a second embodiment crude 540 compound, which has not been treated with a decolorizing agent, is purified by passage over neutral alumina or sephadex.
Claims19
A method for purifying 8-C-.beta.-D-2-O-(E)-cinnamoyl!glycopyranosyl-2-(R)-2-hydroxy!propyl-7-m ethoxy-5-methyl-chromone (the "540 compound"), comprising the steps of:
- (a) contacting a decolorizing agent, which has been obtained from the processing of a slurry of an aloe leaf extract, with an organic solvent to extract the absorbed 540 compound from the decolorizing agent into the organic solvent, thereby forming a mixture of decolorizing agent and organic solvent containing the 540 compound;
- and
- (b) separating the decolorizing agent from the organic solvent to yield a solution highly enriched in compound 540.
A method for purifying compound 540 isolated from the aloe plant comprising the steps of:
- (a) applying a solution of crude aloe extract to a pad of neutral alumina;
- and
- (b) eluting the extract with an organic solvent.
A method for purifying compound 540 isolated from the aloe plant comprising the steps of:
- (a) applying a solution of crude aloe extract to a Sephadex, column;
- and
- (b) eluting the extract with an organic solvent.
Dependencies are inferred from the claim text.