US 5,674,997 AGranted

DNA purification on modified siligates

AssigneeBecton, Dickinson and Company

InventorsDaniel Lee Woodard, Adriann Jeanelle Howard, James Arthur Down

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Abstract

The present invention relates to a silicon-containing material which exhibits sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the material. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the silicon-containing material. Preferred silicon-containing materials of the present invention include boron silicate, aluminum silicate, phosphosilicate, silica carbonyl, silica sulfonyl and silica phosphonyl. The silicon-containing materials of the present invention are particularly useful in processes for purification of DNA from other cellular components. In these processes, a suspension of cellular components is placed in contact with the silicon-containing material, the silicon-containing material is washed to remove all cellular components other than DNA which are bound to the material, and the bound DNA is eluted from the material. Several of the silicon-containing materials are capable of binding and eluting DNA using only water.

Claims3

Dependent claims

A method for purifying DNA comprising the steps of:

(a) contacting a suspension containing DNA with a silicon-containing material which exhibits sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the material with water, said silicon-containing material selected from the group consisting of boron silicate prepared by reacting SiCl.sub.4 with about 0.09 to about 1.5 equivalents of BCl.sub.3 in the presence of water, aluminum silicate prepared by reacting SiCl.sub.4 with about 0.1 to about 0.5 equivalents of AlCl.sub.3 in the presence of water, phosphosilicate prepared by reacting SiCl.sub.4 with about 0.1 to about 5 equivalents of PCl.sub.3 in the presence of water, silica carbonyl prepared by reacting SiCl.sub.4 with about 0.1 to about 1.5 equivalents of COCl.sub.2 in the presence of water, silica sulfonyl prepared by reacting SiCl.sub.4 with about 0.1 to about 1.5 equivalents of SOCl.sub.2 in the presence of water, and silica phosphonyl prepared by reacting SiCl.sub.4 with about 0.1 to about 2 equivalents of POCl.sub.3 in the presence of water under conditions suitable to bind DNA to said material;
(b) washing said material having bound DNA;
and
(c) eluting the bound DNA from said material.

1 more element

Dependencies are inferred from the claim text.