Method for isolation of unclipped HIV envelope protein
AssigneeGenentech, Inc.
InventorsPhillip W. Berman, Brian M. Fendly, Timothy J. Gregory, Florian M. Wurm
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Abstract
Methods for producing and isolating unclipped HIV env proteins are provided. According to the methods, an antibody directed to an HIV epitope spanning the env clip site is used to selectively separate unclipped HIV env protein from clipped env protein.
Claims6
A method for recovering unclipped HIV env which is greater than 50% free of clipped HIV env comprising the following steps:
- a. contacting a first preparation of HIV env with an antibody directed to an HIV env epitope spanning the clip site for a time sufficient to permit formation of a second, antibody-bound unclipped HIV env preparation;
- b. separating the second preparation from any HIV env which is not antibody-bound;
- and
- c. recovering the unclipped HIV env from said second preparation.
A method for the isolation of unclipped HIV env which is greater than 50% free of clipped HIV env, comprising affinity chromatography wherein antibody directed to an HIV env epitope spanning the clip site is bound to a carrier matrix and a solution containing HIV env and unclipped HIV env is passed over the column and unclipped HIV env is selectively adsorbed to the matrix-bound antibody, the adsorbed antibody-unclipped HIV env matrix is washed to remove non-adsorbed material, and the unclipped HIV env is eluted.
Dependencies are inferred from the claim text.