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US 5,674,713 AGranted
DNA sequences encoding coleoptera luciferase activity
AssigneeThe Regents of the University of California
InventorsMarlene DeLuca McElroy, deceased, Donald Raymond Helinski, Keith Vernon Wood, Jeffrey Roux De Wet, David Wing Ow, Stephen Herbert Howell
Life of this patent
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Abstract
DNA compositions and methods of constructing and using the same consisting of DNA sequences encoding luciferase activity, or DNA sequences encoding hybrid molecules exhibiting luciferase activity and a second biological activity that are useful in performing biological assays.
Claims17
A method for producing hybrid proteins
- which comprises expressing in a procaryotic or eucaryotic cell, a lysate, of said cell or a cell-free protein translation system, a first recombinant DNA sequence coding for Coleoptera luciferase;
- expressing a second DNA sequence coding for a second polypeptide having an activity different from that said Coleoptera luciferase, and
- causing transcription and subsequent translation of said first and second DNA sequence as a unit to yield said hybrid protein translation product which catalyzes the oxidation of Coleoptera luciferin to yield light and which also expresses said other activity.
A method for producing hybrid proteins which comprises:
- expressing in a procaryotic or eucaryotic cell, a lysate of said cell or a cell-free protein translation system, a first recombinant RNA sequence coding for Coleoptera luciferase;
- expressing a second RNA sequence coding for a second polypeptide having an activity different from that of said Coleoptera luciferase, and
- causing translation of said first and second RNA sequences as a unit to yield said hybrid protein translation product which catalyzes the oxidation of Coleoptera luciferin to yield light and which also expresses said other activity.
Dependencies are inferred from the claim text.