Episomal vector systems and related methods
OwnerSavio L. C. Woo, Peter W. Nordloh +1inventors
InventorsSavio L. C. Woo, Peter W. Nordloh, Arne Stenlund
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Abstract
An episomal vector sytem and methods for use in gene therapy in humans and animals. The vector contains a papilloma virus origin of replication, a vector maintenance sequence, a first promoter transcriptionally linked to a therapeutic nucleic acid sequence, a second promoter transcriptionally linked to a papilloma virus E1 gene sequence, and a third promoter transcriptionally linked to a papilloma virus E2 gene sequence or an E1/E2 fusion gene. The vector is capable of episomal replication in human or animal cells.
Claims39
An episomal vector system consisting essentially of:
- a papilloma virus origin of replication;
- a first promoter transcriptionally linked to a DNA sequence;
- a second promoter transcriptionally linked to a papilloma virus E1 gene sequence;
- and
- a third promoter transcriptionally linked to a papilloma virus E2 gene sequence;
- and
- wherein said second and third promoters and said E1 and E2 gene sequences are at least about 1 kb from said origin of replication, and said vector comprises no other papillomavirus coding sequences in addition to E1 and E2 gene sequences;
- and
- wherein said episomal vector system comprises one or more episomal vectors, each able to replicate as an episome.
An episomal vector system consisting essentially of:
- a papilloma virus origin of replication;
- a vector maintenance sequence;
- a first promoter transcriptionally linked to a DNA sequence;
- a second promoter transcriptionally linked to an E1/E2 fusion gene sequence and no other papilloma virus coding sequences, said fusion gene sequence containing at least the trans-activation region of the E2 gene sequence;
- and
- wherein said second promoter and said E1/E2 fusion gene sequence is at least about 1 kb from said origin of replication;
- and
- wherein said episomal vector system comprises one or more episomal vectors, each able to replicate as an episome.
Dependencies are inferred from the claim text.