# US 5,674,500 A · Vaccines against Aujeszky's disease and other animal diseases containing pseudorabies virus mutants

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- Number: US 5,674,500 A
- Document ID: US5674500A
- Status: Granted
- Kind: A
- Application number: 08/373,325
- Filed: 1995-01-09
- Granted: 1997-10-07
- Earliest claimed: 1992-07-09
- Assignees: Akzo Nobel N.V.
- Inventors: Bernardus Petrus Hubertus Peeters; Jan Marie Antonius Pol; Arnold Leonard Josef Gielkens; Robertus Jacobus Maria Moormann
- Web page: https://patentgrep.com/patent/US5674500A

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 39/12: Viral antigens
- A61K 39/245: Herpetoviridae, e.g. herpes simplex virus
- A61P 31/12: Antivirals
- C07K 14/005: from viruses
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- A61K 2039/543: {intranasal}
- C12N 2710/16722: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2710/16734: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2710/16761: Methods of inactivation or attenuation
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The invention provides vaccines for preventing and controlling animal diseases, comprising a pseudorabies virus containing glycoprotein gp50 and having a mutation in its gp50 gene. The vaccines are suitable for use against Aujeszky's disease (pseudorabies), or against other animal diseases when the mutation is an insertion comprising a heterologous gene encoding an antigen corresponding to said animal disease. The pseudorabies virus may additionally have at least one mutation in one of its other genes, such as the gp63 gene or the gI gene. The vaccines are unable to spread from vaccinated to non-vaccinated animals.

## Claims

### Claim 1

A vaccine comprising a pseudorabies virus containing glycoprotein gp50 and having a mutation in its gp50 gene, together with a pharmaceutically acceptable carrier or diluent.

### Claim 2 (depends on 1)

A vaccine according to claim 1 wherein the mutation is a deletion.

### Claim 3 (depends on 1)

A vaccine according to claim 1 wherein the mutation is an insertion comprising a heterologous gene encoding an antigen corresponding to an animal disease other than Aujesky's disease.

### Claim 4 (depends on 1)

A vaccine according to any of claim 1, wherein the pseudorabies virus additionally has at least one mutation in one of its other genes.

### Claim 5 (depends on 4)

A vaccine according to claim 4, wherein said at least one additional mutation is in the gp63 gene.

### Claim 6 (depends on 4)

A vaccine according to claim 4, wherein said at least one additional mutation is in the gI gene.

### Claim 7 (depends on 1)

The vaccine according to claim 1, wherein said mutated pseudorabies virus contains an additional mutation at a site other than the gp50 gene, and wherein said additional mutation is an insertion of a heterologous gene that encodes an antigen of an animal pathogen.

### Claim 8 (depends on 1)

A method for preventing and/or controlling an animal disease, comprising administering an immunogenically effective amount of the vaccine according to claim 1.

### Claim 9 (depends on 2)

The method for preventing and/or controlling an animal disease, which comprises administering an immunogenically effective amount of a vaccine according to claim 2.

### Claim 10 (depends on 3)

The method for preventing and/or controlling an animal disease, which comprises administering an immunogenically effective amount of a vaccine according to claim 3.