Method for separating and purifying .alpha.-linolenic acid from perilla oil
AssigneeRepublic of Korea Represented by Rural Development Administration
InventorsSu-Noh Ryu, Jung-Il Lee, Bo-Young Jeong, Han-Sun Hur
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a method for separating and purifying .alpha.-linolenic acid (ALA) from ALA-containing fatty acid mixtures by using a column chromatography, comprises the steps of: packing a column with silver nitrate(AgNO.sub.3)-impregnated silica gel as a stationary phase; passing the ALA-containing fatty acid mixtures through the column to adsorb the fatty acids to the stationary phase in the form of Ag.sup.+ -complexes; eluting the fatty acids with acetone-hexane mixtures; and collecting the fractions containing ALA having a purity of more than 95%.
Claims5
A method for separating and purifying .alpha.-linolenic acid (ALA) from ALA-containing fatty acid mixtures by using a column chromatography, comprises the steps of:
- packing a column with silver nitrate(AgNO.sub.3)-impregnated silica gel as a stationary phase;
- passing the ALA-containing fatty acid mixtures through the column to adsorb the fatty acids to the stationary phase in the form of Ag.sup.+ -complexes;
- eluting the fatty acids with acetone-hexane mixtures;
- and
- collecting the fractions containing ALA having a purity of more than 95%.
Dependencies are inferred from the claim text.