US 5,672,696 A · Treatment of paraffin embedded tissue for gene analysis

Abstract

Rapid and easy preparation of a sample for a gene analysis or high-purity nucleic acid suitable for gene amplification especially, for example, by the PCR method is made possible by a process for treating a paraffin-embedded tissue sample to be used for a gene analysis, which comprises heating an aqueous suspension containing a surfactant having a protein-denaturational action and a deparaffinized tissue sample obtained from a paraffin-embedded tissue sample at 60.degree. C. or higher, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, mixing the resulting reaction solution with a solution containing an organic compound having a protein-denaturational action other than the aforesaid surfactant, and precipitating nucleic acid from the resulting reaction solution by addition of an alcohol.

Claims10

Dependent claims

A process for treating a paraffin-embedded tissue sample to be used for a gene analysis, which comprises

heating an aqueous suspension containing a surfactant having a protein-denaturational action and a deparaffinized tissue sample obtained from a paraffin-embedded tissue sample at 60.degree. C. or higher,
reacting the heat-treated aqueous suspension with a protease,
mixing the resulting reaction solution with a solution containing hydroxybenzoic acid.

Dependencies are inferred from the claim text.

Treatment of paraffin embedded tissue for gene analysis

Life of this patent

Treatment of paraffin embedded tissue for gene analysis

Life of this patent

Abstract

Claims10