Non-cleavable GP160 glycoproteins of HIV
AssigneeTransgene S.A. and Institute Pasteur
InventorsMarie-Paule Kieny, Guy Rautmann, Jean-Pierre Lecocq, Simon Wain Hobson, Marc Girard, Luc Montagnier
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Abstract
A viral vector comprising at least a portion of the genome of the HIV virus, a gene encoding gp160 glycoprotein of the envelope of the HIV virus, as well as the elements providing for the expression of the glycoprotein in cells, wherein the gp160 is expressed as a non-cleavable protein.
Claims23
A non-cleavable gp160 glycoprotein, consisting essentially of gp120-gp40 of a human immunodeficiency virus Type 1 (HIV-1), wherein said gp160 does not contain the amino acid sequence REKR found in natural gp160, and wherein the transmembrane region found in natural gp160 is replaced by the transmembrane region of the glycoprotein of the rabies virus.
A non-cleavable gp160 glycoprotein, consisting essentially of gp120-gp40 of a human immunodeficiency virus Type 1 (HIV-1), wherein said gp160 does not contain the amino acid sequences KRR and REKR found in natural gp160, and wherein the transmembrane region found in natural gp160 is replaced by the transmembrane region of the glycoprotein of the rabies virus.
A non-cleavable gp160 glycoprotein, consisting essentially of gp120-gp40 of a human immunodeficiency virus Type 1 (HIV-1), wherein said gp160 does not contain an amino acid sequence REKR found in natural gp160, further comprising a signal peptide selected from the group consisting of the signal peptide of the precursor of the gp160 of a HIV-1 virus and the signal peptide of the precursor of the glycoprotein of the rabies virus.
A non-cleavable gp160 glycoprotein, consisting essentially of gp120-gp40 of a human immunodeficiency virus Type 1 (HIV-1), wherein said gp160 does not contain an amino acid sequence REKR found in natural gp160, wherein the DNA encoding envelope protein of HIV-1 is encoded by the EcoRI-KpnKI and KpnI-HindIII fragments of plasmid PJ19-13, comprising nucleotides 1258 to 1698 of the DNA encoding envelope protein of HIV-1, and the HindIII-XhoI fragment of plasmid PJ19-6, comprising nucleotides 1698 to 9173 of the DNA encoding envelope protein of HIV-1.
A non-cleavable gp160 glycoprotein, consisting eassentially of gp120-gp40 of a human immunodeficiency virus Type 1 (HIV-1), wherein said gp160 does not contain an amino acid sequence REKR found in natural gp160, wherein said gp160 does not contain the amino acid sequences KRR and REKR found in natural gp160, and wherein the DNA encoding envelope protein of HIV-1 envelope DNA is encoded by the EcoRI-KpnI and KpnI-HindIII fragments of the plasmid PJ19-13, comprising nucleotides 1258 to 1698 of the DNA encoding envelope protein of HIV-1, and the HindIII-XhoI fragment of plasmid PJ19-6, comprising nucletides 1698 to 9173 of the DNA encoding envelope protein of HIV-1.
A non-cleavable gp160 glycoprotein, consisting essentially of gp120-gp40 of a human immunodeficiency virus Type 1 (HIV-1), wherein said gp160 does not contain an amino acid sequence REKR found in natural gp160.
Dependencies are inferred from the claim text.