Source of apolipoprotein E and method of isolating apolipoprotein E
AssigneeDuke University
InventorsWilliam D. Matthew, Warren J. Strittmatter, Catherine R. Gutman
Life of this patent
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Abstract
Peritoneal fluid and peritoneal wash fluid provide a source of abundant apolipoprotein E. Methods for isolating apolipoprotein E from peritoneal fluid or peritoneal wash fluid are provided.
Claims9
A method of isolating apolipoprotein E, comprising:
- (a) providing a sample of human peritoneal fluid or human peritoneal wash fluid;
- (b) isolating apolipoprotein E from said sample.
A method of isolating apolipoprotein E from human peritoneal fluid or human peritoneal wash fluid, comprising the steps of contacting said fluid to Cibacron Blue F3GA dye under conditions so that apolipoprotein E binds to said dye, and then eluting bound apolipoprotein E from said dye.
A method of isolating apolipoprotein E from a sample of human peritoneal fluid or human peritoneal wash fluid comprising the steps of:
- (a) adding fatty acids to a sample of human peritoneal fluid or human peritoneal wash fluid in an amount effective to minimize binding of albumin to Cibacron Blue F3GA dye;
- (b) contacting said sample from step (a) with Cibacron Blue F3GA dye under conditions which allow binding of apolipoprotein E to said dye;
- and
- (c) eluting apolipoprotein E bound to said dye.
Dependencies are inferred from the claim text.