Biocompatability for solid surfaces
AssigneeMedtronic, Inc.
InventorsMichel Verhoeven, Linda L. Cahalan, Marc Hendriks, Benedicte Fouache, Patrick T. Cahalan
Life of this patent
Estimating the term…
Abstract
An improved coating and spacer material for a medical device having a blood or tissue-contacting surface comprising a polyalkyleneimine layer which is crosslinked with a crosslinking agent which is at least difunctional in polymerizable vinyl groups which have adjacent strong electron-withdrawing groups and a biomolecule covalently bonded to the crosslinked polyalkyleneimine layer. For example, polyethyleneimine crosslinked with divinyl sulfone could be used. The resulting crosslinked spacer layer has improved uniformity and stability without materially limiting the covalent attachment of a biomolecule such as heparin.
Claims6
A method for making a medical device having a solid surface for contacting blood or tissue of a patient, the solid surface having applied thereto an immobilized biomolecule by the steps of:
- (a) reacting a polyalkyleneimine with a crosslinking agent which is at least difunctional in polymerizable vinyl groups, each of said polymerizable vinyl groups adjacent a strong electron-withdrawing group, the polyalkyleneimine present in stoichiometric excess in the reaction mixture such that less than about 1/6 of the total amine groups in the polyalkyleneimine are used in the reaction;
- (b) applying the reacted polyalkyleneimine to the solid surface;
- and
- (c) applying a biomolecule having a pendant aldehyde group to the applied polyalkyleneimine such that the pendant aldehyde group of the biomolecule covalently bonds to an amine functionality of the crosslinked polyalkyleneimine.
A method for making a medical device having a solid surface for contacting blood of a patient, the solid surface having applied thereto an immobilized heparin by the steps of:
- (a) reacting a polyalkyleneimine with a crosslinking agent which is at least difunctional in polymerizable vinyl groups, each of said polymerizable vinyl groups adjacent a strong electron-withdrawing group selected from the group consisting of sulfone, carbonyl, azo, sulfamido and nitro groups, the polyalkyleneimine present in stoichiometric excess in the reaction mixture such that less than about 1/6 of the total amine groups in the polyalkyleneimine are used in the reaction;
- (b) applying the reacted polyalkyleneimine to the solid surface;
- (c) treating heparin to produce a pendant aldehyde group on the heparin;
- and
- (d) applying a the treated heparin to the applied polyalkyleneimine such that the pendant aldehyde group of the (d) applying a the treated heparin to the applied polyalkyleneimine such that the pendant aldehyde group of the heparin covalently bonds to an amine functionality of the crosslinked polyalkyleneimine.
Dependencies are inferred from the claim text.