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US 5,672,517 AGranted
Methods and compositions for diagnosis and treatment of interstitial cystitis
OwnerGerald J. Domingueinventor
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Abstract
The present inventor discloses a description of the preparation of ICAPs for use in diagnosis, methods of prevention and prophylaxis of interstitial cystitis. The forms can be cultivated in high concentrations of serum, they can be visualized in a light microscope, they have a characteristic morphology in an electron microscope, and they are immunogenic and antigenic.
Claims8
A process for obtaining an interstitial cystitis associated particle (ICAP), produced by the process of
- obtaining a test sample from a person having symptoms of interstitial cystitis, the test sample being selected from the group consisting of human body fluids, a first serum, a first plasma, and tissue;
- and
- filtering the test sample through a filter, the filter containing pores with a diameter less than or equal to approximately 0.22 mm;
- and
- propagating the test sample in enriched cell free medium effective for growing an interstitial cystitis associated particle;
- and identifying a particle by an effective means, the particle having a core with a diameter of approximately 40-80 nm, the core having sheets continuing therefrom, and the sheets being regularly spaced apart.
3 more elements
A diagnostic assay for interstitial cystitis, the diagnostic assay comprising the process of providing a test sample from a human having the symptoms of interstitial cystitis;
- the samples being selected from the group consisting of human body fluids, serum, plasma, and tissue;
- and
- filtering the samples through a filter containing pores with a diameter less than or equal to approximately 0.22 mm;
- and
- collecting the filtrates;
- and
- disrupting the filtrates sufficiently to release components therefrom;
- and
- sorting the components into an ordered array;
- and
- contacting the components with interstitial cystitis associated particle specific antibodies sufficiently to form immune complexes with the specific antibodies, and wherein the components that form the immune complexes have molecular weights of approximately 28, 58, 68, and 80 kilodaltons;
- and
- detecting the immune complexes by an effective means, whereby a distribution pattern of the immune complexes is produced.
10 more elements
Dependencies are inferred from the claim text.