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US 5,672,508 AGranted
Inhibitors of cell-cycle progression, and uses related thereto
AssigneeMitotix, Inc.
InventorsJeno Gyuris, Lou Lamphere, David Beach
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AssigneeMitotix, Inc.
InventorsJeno Gyuris, Lou Lamphere, David Beach
Estimating the term…
The present invention pertains to novel inhibitors of cyclin-dependent kinases (CDKs), particularly CDK/cyclin complexes, which inhibitors can be used to control proliferation and/or differentiation of cells in which the inhibitors are introduced. More specifically, the inhibitors of the invention are chimeric proteins which include CDK-binding motifs from two or more different proteins. For example, the subject chimeric proteins can be generated from the in-frame fusion of coding sequences from two different CDK inhibitor proteins, such as may be derived from fusion of coding sequences for an INK4 protein and coding sequences for a CIP protein. Chimeric proteins of the present invention have been observed to be more potent inhibitors of cyclin/CDK complexes than were either of the portions of the chimeric protein individually.
A nucleic acid comprising a nucleotide sequence encoding a fusion polypeptide comprising CDK-biding motifs from two or more different proteins which bind to cyclin dependent kinases.
A recombinant transfection system, comprising
A nucleic acid comprising a nucleotide sequence encoding a fusion polypeptide comprising a first CDK-binding motif comprising a polypeptide sequence having tandemly arranged ankyrin-like sequences, and a second CDK-binding motif comprising a polypeptide sequence having a p21/p27 inhibitory domain.
A nucleic acid comprising a nucleotide sequence encoding a fusion polypeptide comprising (i) a polypeptide sequence having a CDK-binding motif of an INK4 protein, and (ii) an polypeptide sequence having a CDK-binding motif of a CIP protein.
A nucleic acid comprising a nucleotide sequence encoding a fusion polypeptide comprising (i) a CDK-binding motif of p16 or p15, and (ii) a p21/p27 inhibitory domain of p21.sup.CIP1, p27.sup.KIP1 or p57.sup.KIP2.
A viral vector comprising a nucleotide sequence encoding a fusion polypeptide comprising CDK-binding motifs from two or more different proteins which bind to cyclin dependent kinases, which viral vector infects mammalian cells and expresses the fusion polypeptide.
An adenoviral vector comprising a nucleotide sequence encoding a fusion polypeptide comprising CDK-binding motifs from two or more different proteins which bind to cyclin dependent kinases.
Dependencies are inferred from the claim text.