Apparatus for the surface culture of nucleated cells and cell culture dependent substances

An apparatus for surface-culture of nucleated cells comprising:

• (a) a vessel for holding nutrient medium and filling bodies on the surfaces of which filling bodies nucleated cells grow during cultivation;
• (b) a cylindrical culture chamber disposed centrally along the vertical axis of the vessel which chamber holds the nutrient medium and filling bodies within the vessel such that the nutrient medium and filling bodies substantially fill the culture chamber but leaving a space at the top of the culture chamber;
• (c) a tube located along the center, vertical axis of the culture chamber having a bottom skirt-like projection, which skirt-like projection is attached to the bottom of the culture chamber and the top of the tube extends towards the top of the culture chamber so as to meet and slightly exceed the level of nutrient medium and filling bodies within the culture chamber, and the surface walls of the tube are perforated so as to allow nutrient medium to pass through but not to allow filling bodies to pass through from the culture chamber, and
• (d) an air diffusion tube disposed along the center vertical axis of the tube having an outlet opening and an inlet opening so that air can be introduced at the bottom of the tube through its outlet opening and then the air can diffuse through the nutrient medium within the tube to oxygenate such nutrient medium and cause the oxygenated nutrient medium to pass through the perforations in the surface walls of the tube and around the filing bodies in the culture chamber, which air diffusion tube's inlet opening is outside of the vessel and its outlet opening is close to the bottom of the tube, whereby the tube together with the air diffusion tube are so arranged that in use air is introduced at the bottom of the culture chamber through the outlet opening of the air diffusion tube and the resulting air bubbles pass up through the annular space between the tube and the air diffusion of the tube to allow the passed air to oxygenate the nutrient medium and to circulate the medium through the filling bodies.