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AssigneeEli Lilly and Company
InventorsKaren L. Cox, Scott E. Fishman, Charles L. Hershberger, Eugene T. Seno
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A method for increasing the antibiotic-producing ability of an antibiotic-producing microbial host cell is disclosed. The method involves transforming an antibiotic-producing microorganism with a recombinant DNA cloning vector that codes for the expression of an antibiotic biosynthetic enzyme or other gene product. The gene preferably codes for a product that is rate-limiting in the antibiotic biosynthetic pathway in Streptomyces. Plasmids that are useful for increasing tylosin production in accordance with the present method include plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315. The invention further comprises microorganisms transformed with plasmids pHJL280, pHJL284, pHJL309, pHJL311, and pHJL315 and also other microorganisms and vectors used in accordance with the present method.
The DNA sequence that encodes a gene product selected from the group consisting of the Streptomyces fradiae tylC, tylD, tylE, tylF, tylH, tylJ, tylK, tylL, and tylM biosynthetic gene products.
A DNA sequence that encodes the translational-activating sequence of a gene selected from the group consisting of the Streptomyces fradiae tylC, tylD, tylE, tylF, tylH, tylJ, tylK, tylL and tylM biosynthetic genes.
A plasmid that is selected from the group consisting of plasmids pSKC13, pSKC16, pSFH60, pSFH61, pSFH62, pOJ163, pOJ190, and pHJL289.
A DNA sequence that encodes the Streptomyces fradiae tylF gene product, said product comprising the amino acid residue sequence:
A DNA sequence encoding the Streptomyces fradiae tylE gene product, said product comprising the amino acid sequence:
A DNA sequence encoding the Streptomyces fradiae tylD gene product, said product comprising the amino acid sequence:
A DNA sequence encoding the Streptomyces fradiae tylH gene product, said product comprising the amino acid sequence:
A DNA sequence encoding the Streptomyces fradiae tylJ gene product, said product comprising the amino acid sequence:
Dependencies are inferred from the claim text.