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US 5,672,485 AGranted
Immortalized cell lines for virus growth
AssigneeRegents of the University of Minnesota
InventorsDouglas N. Foster, Linda K. Foster
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Abstract
This invention relates to the production and use of immortalized cell lines from primary chicken embryonic fibroblasts. The cells are useful as substrates for virus propagation, recombinant protein expression and recombinant virus production.
Claims9
A spontaneously immortalized cell line, derived from primary chicken embryonic fibroblasts, having the characteristics of UMNSAH-DF 1 cell line and deposited with the American Type Culture Collection as Accession #CRL-12203.
A method for producing an immortalized cell line from chicken embryonic fibroblasts comprising the steps of:
- growing primary chicken embryonic fibroblasts in culture;
- passaging the fibroblasts in culture until they begin cell senescence;
- concentrating the cells during cell senescence to maintain about 30% to about 60% culture confluence;
- identifying foci of non-senescent cells in the culture;
- isolating the non-senescent cells;
- and
- growing the non-senescent cells for greater than 30 passages.
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