Method for purifying cyclic inulooligosaccharide
AssigneesMitsubishi Chemical Corporation, Mitsubishi Kasei Engineering Company
InventorsSachiko Kushibe, Masao Tamura
Life of this patent
Estimating the term…
Abstract
An exo-enzyme having an ability to cut .beta.-(2.fwdarw.1) fructoside bond or a microorganism which produces the enzyme is allowed to act on a saccharide solution containing cyclic inulooligosaccharide in which fructose molecules are bonded through .beta.-(2.fwdarw.1) bond in a cyclic configuration, and other saccharides such as monosaccharide, disaccharide, linear oligosaccharide and inulin, and then the cyclic inulooligosaccharide is collected from an obtained saccharide solution. Thus cyclic inulooligosaccharide can be purified inexpensively at a high yield without using any solvent.
Claims6
A method for purifying cyclic inulooligosaccharide, comprising the steps of:
- allowing an exo-enzyme having an ability to cut .beta.-(2.fwdarw.1) fructoside bond or a microorganism which produces the exo-enzyme to act on a saccharide solution containing (a) cyclic inulooligosaccharide in which fructose molecules are bonded through .beta.-(2.fwdarw.1) bond in a cyclic configuration, and (b) at least one saccharide selected from disaccharide, linear oligosaccharide and inulin, said enzyme decomposing said saccharide into monosaccharide, disaccharide, or both;
- and
- subsequently collecting the cyclic inulooligosaccharide from the thus obtained saccharide solution.
Dependencies are inferred from the claim text.