White blood cell hematology control
AssigneeStreck Laboratories, Inc.
InventorWayne L. Ryan
Life of this patent
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Abstract
Reference controls comprised of aldehyde-fixed white blood cells stabilized red blood cells and simulated blood platelets exhibiting a white blood cell histogram profile that is substantially that of whole blood are obtained by the addition of a lipoprotein to the control and an antioxidant to inhibit lysis of stabilized red blood cells by the lipoprotein.
Claims42
A reference control composition for hematology instruments comprising:
- a) fixed or stabilized red blood cells as a white blood cell surrogate, suspended in an isotonic aqueous suspension medium;
- and
- b) a lipoprotein in an amount sufficient to provide a composition which gives a white blood cell histogram profile that is substantially that of whole blood.
A method for making a reference control composition for hematology instruments comprising the step of mixing fixed or stabilized red blood cells as a white blood cell surrogate, in an isotonic aqueous suspension medium, and a lipoprotein, wherein the amount of lipoprotein is an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood.
A reference control composition for hematology instruments comprising:
- a) white blood cells fixed or stabilized and suspended in an isotonic aqueous suspension medium;
- and
- b) a lipoprotein in an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood.
A method for making a reference control composition for hematology instruments comprising the step of mixing fixed or stabilized white blood cells, suspended in an isotonic aqueous suspension medium, and a lipoprotein, wherein the amount of lipoprotein is an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood.
A method of determining the accuracy of a hematology instrument comprising the steps of:
- a) providing a reference control comprising a blood cell control means suitable as a reference control for blood, wherein the blood cell control means is fixed or stabilized and suspended in an isotonic aqueous suspension medium, and a lipoprotein in an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood;
- b) passing the reference control through the hematology instrument to provide a histogram of the reference control;
- and
- c) comparing the histogram of step b) with assayed values of the reference control.
A method of determining the accuracy of a hematology instrument comprising the steps of:
- a) providing a reference control comprising a blood cell control means suitable as a reference control for blood, wherein the blood cell control means is fixed or stabilized and suspended in an isotonic aqueous suspension medium, and a composition comprising lipoprotein, wherein the lipoprotein is present in an amount sufficient to provide a composition that gives a white blood cell histogram profile that is substantially that of whole blood;
- b) passing the reference control through the hematology instrument to provide a histogram of the reference control;
- and
- c) comparing the histogram of step b) with assayed values of the reference control.
Dependencies are inferred from the claim text.