Recombinant bovine coronavirus E2 and E3 polypeptides and vaccines
AssigneeVeterinary Infectious Disease Organization
InventorsMichael D. Parker, Graham J. Cox, Lorne A. Babiuk
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Abstract
Nucleic acid sequences encoding the Bovine Coronavirus E2 (or BCV S) and E3 (or BCV HE) structural glycoproteins and methods of producing these proteins, including recombinant expression, e.g., in mammalian or insect cells, are provided. The E2 and E3 proteins or antigenic fragments thereof are useful components for Bovine Coronavirus vaccines and methods of treatment.
Claims18
A method of eliciting an immune response which reduces the severity of Bovine coronavirus (BCV) infection in a mammalian host, said method comprising:
- (a) providing a vaccine composition comprising a pharmaceutically acceptable carrier and at least one recombinant BCV polypeptide comprising a BCV neutralization epitope selected from the group consisting of E2 (S) having a molecular weight ranging from approximately 170 to 200 kDa, E3 (HE) having a molecular weight of approximately 120-140 kDa, a fragment of E2 and a fragment of E3;
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- (b) administering to the mammalian host an amount of the vaccine composition effective to elicit an immune response which reduces the severity of Bovine coronavirus infection.
A vaccine composition for reducing the severity of bovine coronavirus (BCV) infection in a mammalian host comprising a pharmaceutically acceptable carrier and an effective amount of at least one recombinant BCV polypeptide comprising a BCV neutralization epitope selected from the group consisting of E2 (S) having a molecular weight ranging from 170 to 200 kDa, E3 (HE) having a molecular weight of approximately 120-124 kDa, a fragment of E2 and a fragment of E3.
A composition comprising a pharmaceutically acceptable carrier and a recombinant BCV polypeptide comprising a BCV neutralization epitope selected from the group consisting of E2 (S) having a molecular weight ranging from 170-200 kDa, E3 (HE) having a molecular weight ranging from 120-124 kDa, a fragment of E2 and a fragment of E3.
Dependencies are inferred from the claim text.