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US 5,672,344 AGranted
Viral-mediated gene transfer system
AssigneeThe Regents of the University of Michigan
InventorsWilliam N. Kelley, Thomas D. Palella, Myron Levine
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AssigneeThe Regents of the University of Michigan
InventorsWilliam N. Kelley, Thomas D. Palella, Myron Levine
Estimating the term…
A recombinant neurotropic HSV-1 vector carrying the gene for human HPRT under the direction of the viral TK promoter expresses a high level of human enzyme in host neuronal cells, yet has reduced cytopathicity. The virulence of the HSV-1 vector is further reduced by rendering it replication-defective through UV-irradiation or the use of a replication-defective HSV-1 deletion mutant in its construction. The recombinant vector is also provided with a non-vital site of initiation of DNA synthesis, which permits the HPRT gene to replicate independently of the viral genome, thus providing means for amplification of the gene in the host. The recombinant HSV-1 vector's high level of expression of HPRT coupled with decreased virulence allows treatment of HPRT deficieny by direct infection of the host organism.
A vector for direct delivery of a gene to a mammalian host comprising a recombinant viral vector which targets and expresses a mammalian gene in a selected cell in the mammalian host, the recombinant vector further comprising:
A method of targeted viral-mediated transfer and expression of a nonviral gene in a selected cell of a host organism comprising the steps of:
A recombinant viral vector which expresses a mammalian gene in a selected mammalian host cell comprising:
A method of viral-mediated transfer and expression of a mammalian gene in a selected cell of a mammalian host organism comprising the steps of:
Dependencies are inferred from the claim text.