Method for fabricating microparticles in emulsion by modification of the chemical composition of the dispersed phase after emulsification
AssigneeColetica
InventorsIsabelle Orly, Marie-Christine Levy, Eric Perrier
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Abstract
The method for producing microparticles is characterized in that it comprises the following steps: preparation of an emulsion of a solution of a substance or a mixture of substances in a dispersing liquid wherein said substance or said mixture are substantially insoluble; incorporating to said dispersed phase a chemical agent substantially insoluble in the dispersing liquid so as to cause within the dispersed phase a chemical or physicochemical reaction responsible for the formation of microparticles which are then isolated.
Claims72
A process for the manufacture of microparticles comprising:
- a) preparing an essentially homogenous solution of a substance or mixture of substances in a first solvent,
- b) producing an emulsion of this homogenous solution in a dispersing liquid forming a continuous phase, in which said substance or said mixture of substances is essentially insoluble, and forming a disperse phase,
- c) initiating a chemical or physicochemical reaction in the disperse phase by modification of the in situ chemical composition of said substance or said mixture or substances in the disperse phase by adding thereto an agent which is essentially insoluble in or immiscible with the continuous phase, under the conditions of addition, thereby modifying the physicochemical state of said disperse phase resulting in the insolubilization of the substance or mixture of substances in the disperse phase and the individualization of said microparticles, in the presence of the first solvent.
A process for the manufacture of microparticles, comprising:
- a) preparing an aqueous solution of a substance selected from the group consisting of nucleic acids, a protein, a polysaccharide and any mixture thereof;
- b) providing a hydrophobic liquid in which said substance or substances are essentially insoluble;
- c) mixing the hydrophobic liquid and the aqueous solution to form an emulsion in which the aqueous solution is a disperse phase and the hydrophobic liquid is a continuous phase;
- d) adding to the emulsion a non-solvent for the substance solubilized in the disperse phase in determined proportions for having said non-solvent essentially immiscible with the continuous phase and for forming microparticles from the disperse phase by insolubilization of said substance or substances in the disperse phase, and
- e) recovering said microparticles by physical separation.
A process for the manufacture of microparticles, comprising:
- a) preparing an aqueous solution of a substance selected from the group consisting of nucleic acids, a protein, a polysaccharide and any mixture thereof, said aqueous solution having a pH adapted for forming an essentially homogeneous solution of said substance or mixture of substances with a viscosity compatible with the production of an emulsion;
- b) providing a hydrophobic liquid in which the substance or mixture of substances are essentially insoluble;
- c) mixing the hydrophobic liquid and the aqueous solution to form an emulsion in which the aqueous solution is a disperse phase and the hydrophobic liquid is a continuous phase;
- d) adding to the emulsion a solution of a substance selected from the group consisting of an alkaline substance and an acid substance, in an organic liquid miscible wit the aqueous phase, thereby insolubilizing said substance or substances resulting in formation of microparticles from said disperse phase;
- and
- e) recovering said microparticles.
Microparticles made from a substance or mixture of substances which has been first solubilized in a first solvent dispersed in a dispersion liquid to form an emulsion before being insolubilized to form said microparticles.
Dependencies are inferred from the claim text.