Process for obtaining an isoflavone concentrate from a soybean extract
AssigneeACDS Technologies, Ltd.
InventorAbas Chaihorsky
Life of this patent
Estimating the term…
Abstract
A process is disclosed for recovering isoflavones from a soy extract comprises: dripping the soy extract dissolved in the aqueous solvent through a chromatographic column from top to bottom packed with a ground highly polar cationic exchange resin containing sulfonic acid functional groups of the formula: {MHSO.sub.3 }--Na wherein MH is the particular adsorbent resin on which the sulfonic acid functional groups are immobilized and wherein the resin is charged with sodium ions which replace the hydrogen ions of sulfonic acid, to selectively adsorb the 7-glycosyl-isoflavones directly on the sulfonate sulfur atom while the undesired proteins and glycosides other than the 7-glycosyl-isoflavones are eluted through the chromatographic column and are removed.
Claims4
A process for recovering isoflavones from a soy extract which comprises the following steps:
- (a) obtaining a defatted, deproteinized soy product which is a soy molasses or soy solubles alcohol-soluble product which contains the desired isoflavones, principally in the form of their 7-glycosides as well as undesired proteins and glycosides other than the desired 7-glycosyl-isoflavones;
- (b) dissolving the soy extract containing the 7-glycosyl-isoflavones and undesired proteins and glycosides other than the desired 7-glycosyl-isoflavones in an aqueous solvent;
- (c) dripping the soy extract dissolved in the aqueous solvent through a chromatographic column from top to bottom packed with a ground highly polar cationic exchange resin containing sulfonic acid functional groups of the formula:
- {MHSO.sub.3 }--Na
- wherein MH is the particular adsorbent resin on which the sulfonic acid functional groups are immobilized and wherein the resin is charged with sodium ions which replace the hydrogen ions of sulfonic acid, to selectively adsorb the 7-glycosyl-isoflavones directly on the sulfonate sulfur atom while the undesired proteins and glycosides other than the 7-glycosyl-isoflavones are eluted through the chromatographic column and are removed;
- and
- (d) desorbing the 7-glycosyl-isoflavones from the chromatographic column packed with a ground highly polar cationic exchange resin containing sulfonic acid functional groups by eluting the chromatographic column with an acidic aqueous alkanol containing 1 to 3 carbon atoms to replace the 7-glycosyl-isoflavones bonded to the sulfonic acid functional groups with hydrogen ions.
Dependencies are inferred from the claim text.