Procedure of separation of proteins by column chromatography using silica gels coated by a lipid bilayer
AssigneesThomas Bayerl, Sybille Bayerl
InventorsThomas Bayerl, Sybille Bayerl
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Abstract
A chromatography column packing material and column chromatographic methods using a silica gel based column packing material are disclosed. The silica gel material is coated by a phospholipid bilayer containing at least two lipids, at least one having an electric charge. Proteins are separated using this column packing material without denaturing because of the lipid coating. The protein molecule binds to the lipid bilayer coated silica gel and is then eluted by raising the temperature.
Claims17
A process for separating proteins according to their molecular weight or their electrical charge state while protecting the proteins against denaturation comprising:
- coating a silica or silicon-based solid substrate with a lipid layer,
- contacting a sample containing proteins with the lipid layer coated silica or silicon-based solid substrate, and
- eluting at least some of the proteins from the lipid layer coated silica or silicon-based solid substrate, wherein individual proteins are separated based upon their interaction with the lipid layer coated solid silica or silicon-based substrate.
In a chromatography process for separating proteins according to their molecular weight or their electrical charge state while protecting the proteins against denaturation comprising contacting a sample containing proteins with a silica or silicon-based solid substrate, and eluting the proteins from the silica or silicon-based solid substrate, wherein individual proteins are separated based upon their interaction with the silica or silicon-based solid substrate, the improvement comprising coating the silica or silicon-based solid substrate with a lipid bilayer before contacting a sample containing proteins with the coated silica or silicon-based solid substrate.
Dependencies are inferred from the claim text.