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US 5,670,630 AGranted
Expression of hepatitis B S and preS.sub.2 proteins in methylotrophic
AssigneeResearch Corporation Technologies, Inc.
InventorGregory P. Thill
Life of this patent
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Abstract
A process for the enhanced production of antigenic particles consisting essentially of hepatitis B S protein and preS.sub.2 protein. Also disclosed are novel DNA molecules and hosts transformed with these molecules.
Claims3
Antigenic Hepatitis B virus (HBV) particles comprising HBV S and preS2 proteins made by a process which comprises:
- (a) transforming a Pichia pastoris strain with a first expression cassette comprising a structural gene for a hepatitis B virus S protein operably linked to a 5' regulatory region and a 3' termination sequence obtainable from Pichia pastoris;
- and
- (b) transforming said Pichia pastoris strain with a second expression cassette comprising a structural gene for the hepatitis B virus preS.sub.2 protein operably linked to a 5' regulatory region and a 3' termination sequence obtainable from Pichia pastoris;
- and
- (c) culturing the resulting transformed Pichia pastoris strain under suitable conditions to obtain the production of said HBV particles.
2 more elements
Recombinantly produced HBV particles consisting of S and preS.sub.2 proteins.
Hepatitis B virus (HBV) particles comprising S and preS.sub.2 proteins produced in a methylotrophic yeast.
Dependencies are inferred from the claim text.