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AssigneeShinwa Chemical Industries, Ltd.
InventorsJun Haginaka, Hiroo Wada, Hiroya Fujima
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An ovoglycoprotein has a molecular weight of 30,000 daltons which is determined by a matrix-assisted laser desorption ionization time-of-flight type mass spectrometer, an amino acid sequence of 15 residues from the N-terminal represented by Thr-Glu-Ser-Pro-Xaa-Ser-Ala-Pro-Leu-Val-Pro-Ala-Asp-Met-Asp (wherein Xaa represents a cysteine residue or an amino acid residue having a sugar chain linked thereto) (SEQ ID NO:1) and a sugar content of about 25% by weight and free of trypsin-inhibitory activity and a resolution agent for chromatography comprises a fixed phase which comprises a carrier and the foregoing ovoglycoprotein linked to the carrier. The agent for resolving optical isomers is not expensive, and is stable and highly resistant to deterioration by organic solvents, excellent in liquid-flow through properties, has a high sample-loading rate and permits efficient resolution of optical isomers of a chiral compound.
An ovoglycoprotein having a molecular weight of about 30,000 daltons as determined by a matrix-assisted laser desorption ionization time-of-flight type mass spectrometer, an amino acid sequence of 15 residues from the N-terminal represented by Thr-Glu-Ser-Pro-Xaa-Ser-Ala-pro-Leu-Val-Pro-Ala-Asp-Met-Asp, (SEQ ID NO:1) wherein Xaa represents a cysteine residue or an amino acid residue having a sugar chain linked thereto, and a sugar content of about 15% hexosamine, about 7% hexose and about 3% sialic acid by weight and free of trypsin-inhibitory activity.
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