Metal ion-ligand coordination complexes, antibodies directed thereto, and assays using such antibodies
AssigneeAbbott Laboratories
InventorDavid K. Johnson
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Abstract
The present invention relates to the field of immunoassays for metal ions. The invention presents: metal ion-ligand coordination complexes ("MLC"), novel ligands, antibodies specific for MLC, immunoassays utilizing the foregoing, and methods for selecting said antibodies.
Claims10
A monoclonal antibody that is specific for an MLC composed of at least two ligands and a metal ion wherein the epitope recognized by the monoclonal antibody comprises two or more ligands in the MLC, wherein the ligands are selected from the group consisting of ##STR1## ##STR2## Where r is selected from the group consisting of --CH.sub.2 -- --(CH.sub.2).sub.2 -- and --(C(CH.sub.3).sub.2)--Y and Z can be the same or different and are selected from the group consisting of --H --CO.sub.2 H and --SO.sub.3 H
- X is selected from the group consisting of --OH --OR.sub.1 --NHR.sub.2 and --NHR.sub.3, wherein the ligands in the MLC can be the same or different provided that Y cannot be H and X cannot be OH when r is (CH.sub.2).sub.2 in Formula L.sub.2, further provided that r cannot be CH.sub.2 or (CH.sub.2).sub.2 in Formula L.sub.5, further provided that r cannot be CH.sub.2 when X is OH in Formula L.sub.6, and further provided that r cannot be CH.sub.2 in Formula L.sub.8.
An assay kit comprising:
- (a) a container containing a ligand selected from the group consisting of ##STR3## ##STR4## Where r is selected from the group consisting of --CH.sub.2 ----(CH.sub.2).sub.2 -- and --(C(CH.sub.3).sub.2)--Y and Z can be the same or different and are selected from the group consisting of --H --CO.sub.2 H and --SO.sub.3 H
- Y is selected from the group consisting of --OH --OR.sub.1 --NHR.sub.2 and --NHR.sub.3 provided that Y cannot be H and X cannot be OH when r is (CH.sub.2).sub.2 in Formula L.sub.2, further provided that r cannot be CH.sub.2 or (CH.sub.2).sub.2 in Formula L.sub.5, further provided that r cannot be CH.sub.2 when X is OH in Formula L.sub.6, and further provided that r cannot be CH.sub.2 in Formula L.sub.8 ;
- and
- (b) a container containing an antibody specific for an MLC comprising a metal ion bound to at least two ligands selected from the group consisting of ##STR5## ##STR6## Where r is selected from the group consisting of --CH.sub.2 -- --(CH.sub.2).sub.2 -- and --(C(CH.sub.3).sub.2)--Y and Z can be the same or different and are selected from the group consisting of --H --CO.sub.2 H and --SO.sub.3 H
- X is selected from the group consisting of --OH --OR.sub.1 --NHR.sub.2 and --NHR.sub.3 provided that Y cannot be H and X cannot be OH when r is (CH.sub.2).sub.2 in Formula L.sub.2, further provided that r cannot be CH.sub.2 or (CH.sub.2).sub.2 in Formula L.sub.5, further provided that r cannot be CH.sub.2 when X is OH in Formula L.sub.6, and further provided that r cannot be CH.sub.2 in Formula L.sub.8, wherein the antibody recognizes an epitope comprising at least two of the ligands in the MLC.
Dependencies are inferred from the claim text.