Insulin-like peptide
AssigneeThe State University of New Jersey
InventorMaureen A. McKenzie
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Abstract
A purified insulin-like protein (ILP) isolated from Saccharomyces cerevisiae is disclosed. The ILP has a molecular weight of approximately 6.4 kilodaltons and the amino acid sequence of the 22 amino terminal residues of the protein has been determined. Computer-assisted molecular graphics analysis of ILP illustrates the similarity of the sequenced portion to vertebrate insulin A-chains.
Claims11
A purified protein which comprises a polypeptide that binds specifically to a yeast insulin-like protein receptor and which is isolated from a yeast culture, wherein said protein has a molecular weight of 6400.+-.200 daltons, as determined by gel filtration chromatography.
A purified protein which comprises an amino acid sequence that is VGVWPTDCSHYAAEKAALQTYCN (SEQ. ID. NO.:11), AGVWPTDCSHYAAEKAALQTYCN (SEQ. ID. NO.:12), VGVWPTDCSHYACAEKAALQTYCN (SEQ. ID. NO.:13), VGVWPTDCSHYAAESAALQTYCN (SEQ. ID. NO.:14), VGVWPTDCSHYAAELAALQTYCN, (SEQ. ID. NO.:15), VGVWPTDCSHYAAEKAALQTYPN (SEQ. ID. NO.:16), AGVWPTDCSHYACAEKAALQTYCN (SEQ. ID. NO.:17), AGVWPTDCSHYAAESAALQTYCN (SEQ. ID. NO.:18), AGVWPTDCSHYAAELAALQTYCN (SEQ. ID. NO.:19), AGVWPTDCSHYAAEKAALQTYPN (SEQ. ID. NO.:20 ), VGVWPTDCSHYACAESAALQTYCN (SEQ. I.D. NO.:21), VGVWPTDCSHYACAELAALQTYCN (SEQ. ID. NO.:22), AGVWPTDCSHYACAESAALQTYCN (SEQ. ID. NO.:23), AGVWPTDCSHYACAELAALQTYCN (SEQ. ID. NO.:24), VGVWPTDCSHYACAEKAALQTYPN (SEQ. ID. NO.:25), AGVWPTDCSHYACAEKAALQTYPN (SEQ. ID. NO.:26), VGVWPTDCSHYACAESAALQTYPN (SEQ. ID. NO.:27), VGVWPTDCSHYACAELAALQTYPN (SEQ. ID. NO.:28), AGVWPTDCSHYACAESAALQTYPN (SEQ. ID. NO.:29), AGVWPTDCSHYACAELALQTYPN (SEQ. ID. NO.:30), VGVWPTDCSHYAAESAALQTYPN (SEQ. ID. NO.:31), VGVWPTDCSHYAAELAALQTYPN (SEQ. ID. NO.:32), AGVWPTDCSHYAAESAALQTYPN (SEQ. ID. NO.:33) or AGVWPTDCSHYAAELAALQTYPN (SEQ. ID. NO.:34), wherein said protein binds specifically to a yeast insulin-like protein receptor, which is isolated from a yeast culture, which has a molecular weight of 6400.+-.200 daltons, which upon binding to said yeast insulin-like protein receptor causes phosphorylation on a tyrosine residue of said yeast insulin-like protein receptor, and which elicits calcium mobilization upon binding to said insulin-like protein.
A purified protein which comprises the amino acid sequence VGVWPTDCSHYAAEKAALQTYCN (SEQ. I.D. NO. 11) and has an apparent molecular weight of 6400.+-.200 daltons when measured by gel filtration chromatography.
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