Method and test kit for the qualitative determination of peroxides in fat

A process for determining whether a fat sample contains fat peroxides in an amount above a predetermined fat peroxides amount comprising the steps of:

• (a) dissolving a predetermined weight of fat to be assayed in a predetermined amount of a 3:2 volume:volume solution of acetic acid:isooctane that also contains a dispersing amount of a surfactant to form a first organic solution;
• (b) agitatingly admixing a predetermined amount of a saturated solution of potassium iodide with said first organic solution to form a second organic solution, the amount of said saturated potassium iodide solution being sufficient to provide an amount of iodide ions needed to react with fat peroxides present in the fat in excess of a predetermined fat peroxides amount to form iodine;
• (c) maintaining said second organic solution with agitation for a time sufficient for the fat peroxides present in the fat to react with iodide ions to form iodine;
• and
• (d) agitatingly admixing an aqueous composition of a starch indicator dispersion in a predetermined amount of iodine reductant solution with said maintained second organic solution to form a two phase, aqueous/organic composition, the predetermined amount of iodine reductant present being sufficient to reduce only an amount of iodine formed from a predetermined amount of fat peroxides that may be present in the fat, an excess of iodine present in said two phase composition causing the aqueous phase to be other than colorless or yellow and indicating that the peroxide value of the fat sample is above the predetermined fat peroxides amount.