Pluripotential embryonic stem cells and methods of making same
AssigneeVanderbilt University
InventorBrigid L. M. Hogan
Life of this patent
Estimating the term…
Abstract
The present invention provides a non-mouse pluripotential embryonic stem cell which can be maintained on feeder layers for at least 20 passages and give rise to embryoid bodies and multiple differentiated cell phenotypes in monolayer culture. The invention further provides a method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Claims1
A method of screening factors for the ability to promote the formation of embryonic stem cells, comprising combining primordial germ cells with a factor selected from the group consisting of fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor with the factor to be screened and determining the formation of embryonic stem cells, the formation of embryonic stem cells indicating a factor capable of promoting the formation of embryonic stem cells.
Dependencies are inferred from the claim text.