Identification of, and uses for, nematicidal bacillus thuringiensis genes, toxins, and isolates
AssigneeMycogen Corporation
InventorJerald S. Feitelson
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Abstract
Disclosed and claimed are novel nucleotide primers for the identification of genes encoding toxins active against nematodes and coleopterans. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The primers are also useful as nucleotide probes to detect the toxins-encoding genes. The subject invention also concerns novel isolates, toxins, and genes useful in the control of plant pests.
Claims23
A novel Bacillus thuringiensis isolate selected from the group consisting of PS32B (NRRL B-21531), PS 49C (NRRL B-21532), PSS52E3 (NRRL B-21533), PS54G2 (NRRL B-21543), PS101CC3 (NRRL B 21534), PS178D4 (NRRL B-21544), PS185L2 (NRRL B-21535), PS197P3 (NRRL B-21536), PS242B6 (NRRL B-21537), PS242G4 (NRRL B-21538), PS242H10 (NRRL B-21539), PS242K17 (NRRL B-21540), PS244A2 (NRRL B-21541), PS244D1 (NRRL B-21542).
An isolated polynucleotide sequence which encodes a nematicidal toxin from a Bacillus thuringiensis isolate selected from the group consisting of PS32B, PS49C, PS53E3, PS54G2, PS101CC3, PS178D4, PS185L2, PS197P3, PS242B6, PS242G4, PS242H10, PS242K17, PS244A2, and PS244D1.
An isolated polynucleotide sequence which is SEQ ID NO. 4.
Dependencies are inferred from the claim text.