Peroxidase produced by plant cell cultures
AssigneePhytera, Inc.
InventorsGagik Stepan-Sarkissian, Debbie Grey, Margaret Elizabeth Spencer, Angela Marian Stafford, Sean Michael Vincent Ashton, Sandra Jane Scollick
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Abstract
A process for the production of peroxidase enzyme comprising an undifferentiated liquid suspension culture of peroxidase-producing plant cells from a plant of the species Theobroma cacao, Coleus blumei, and Silene alba, wherein the culture medium contains a carbohydrate waste material, and peroxidase enzymes derived therefrom.
Claims3
A substantially purified peroxidase isoenzyme, wherein the isoenzyme is PT-S3, which is an unglycosylated, extracellular isoenzyme obtained from Theobroma cacao, having a molecular weight of about 33 kD, an optimum pH of 4.5, a haem peak of 403 nm, a R.sub.z of 2.05, a pI value greater than 10, a highest specific activity measured with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) of 12046 U/mg, and a highest specific activity measured with guaiacol of 3200 U/mg;
- and wherein the enzyme retains more than 33% activity after 1 year at 4.degree. C. in sodium acetate or phosphate buffer.
A substantially purified peroxidase isoenzyme, wherein the isoenzyme is PT-S2, which is an extra-cellular isoenzyme obtained from Theobroma cacao, having a molecular weight of about 36 kD, an optimum pH between 5 and 6, a haem peak of 419 nm, a R.sub.z of 1.9, a pI value greater than 10, a highest specific activity measured with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) of 3200 U/mg, and a highest specific activity measured with guaiacol of 17800 U/mg.
A substantially purified peroxidase isoenzyme, wherein the isoenzyme is PSA-S, which is an extra-cellular isoenzyme obtained from Silene alba, having a molecular weight of about 35 kD, an optimum pH of 4.5, a haem peak of 405 nm, a R.sub.z of 3.8, a pI value of 9.5, and a highest specific activity measured with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) of 7040 u/mg.
Dependencies are inferred from the claim text.