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US 5,670,341 AGranted
DNA vectors, hosts, probes and recombinant methods of preparing human somatomedin-like carrier proteins or polypeptides
AssigneeCeltrix Pharmaceuticals, Inc.
InventorsEmerald Martin Spencer, Carol Talkington-Verser
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Abstract
Methods and compositions are disclosed for producing human somatomedin carrier protein-like polypeptides. Human somatomedin carrier protein-like polypeptides, which bind somatomedins, are produced using recombinant technology. Also disclosed are methods for making complexes of human somatomedin carrier protein-like polypeptides and somatomedins.
Claims20
A recombinant DNA molecule comprising:
- a first DNA sequence encoding amino acids 27-290 of FIG. 4, wherein said first DNA sequence is preceded by a second DNA sequence encoding a secretion or signal sequence not normally found in a gram-negative bacterial host cell, such that translation is initiated at the beginning of or upstream of said second DNA wherein said second DNA is operably linked to an expression control sequence of a bacterial origin, and wherein the polypeptide encoded by said sequence accumulates inside said gram-negative bacterial host cell in soluble form.
A recombinant DNA molecule comprising:
- a DNA construct, said DNA construct comprising a first DNA sequence encoding amino acids 27-290 of FIG. 4 and a second DNA sequence encoding a signal sequence from preproinsulin, said second DNA sequence preceding said first DNA sequence and said second DNA sequence fused in frame to said first DNA sequence;
- and an expression control sequence of bacterial origin, wherein said expression control sequence is operably linked to said DNA construct and wherein expression of said DNA construct in a host cell results in the accumulation of a soluble protein within said host cell, said soluble protein comprising the amino acid sequence of amino acids 27-290 of FIG. 4.
Dependencies are inferred from the claim text.