DNA encoding single chain monellin
AssigneesThe Regents of the University of California, Lucky Biotech Corp.
InventorsSung-Hou Kim, Joong Myung Cho
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Abstract
Single-chain analogs of the naturally occurring two-chain peptide monellin retain the sweetening properties of the natural protein and are stable under conditions which would otherwise destabilize the native peptide. A covalent linkage joins peptides corresponding to portions of the A and B chains of the naturally occurring protein.
Claims12
A synthetic or recombinant DNA in purified and isolated form which encodes a single-chain sweet proteinaceous compound of the formula B-C-A,
- wherein B represents a peptide portion consisting of gene-encoded amino acids which is at least 90% homologous to residues 1-45 of the B chain of native monellin and modified only by conservative substitutions;
- C is a covalent bond or consists of a peptide of 1-10 gene-encoded amino acids;
- and
- A represents a peptide portion consisting of gene-encoded amino acids at least 90% homologous to residues 6-45 of the A chain of native monellin and modified only by conservative substitutions.
An expression system effective, when contained in a host cell to produce a protein useful as a sweetener of the formula B-C-A,
- wherein B represents a peptide portion consisting of gene-encoded amino acids which is at least 90% homologous to residues 1-46 of the B chain of native monellin and modified only by conservative substitutions;
- C is a covalent bond or consists of a peptide of 1-10 gene-encoded amino acids;
- and
- A represents a peptide portion consisting of gene-encoded amino acids at least 90% homologous to residues 6-45 of the A chain of native monellin and modified only by conservative substitutions;
- which expression system comprises an oligonucleotide sequence encoding said protein operably linked to control sequences compatible with the host cell.
Dependencies are inferred from the claim text.