Polymorphism of human platelet membrane glycoprotein IIIa and diagnostic and therapeutic applications thereof
AssigneeBlood Center Research Foundation
InventorsPeter J. Newman, Richard H. Aster
Life of this patent
Estimating the term…
Abstract
Isolated polynucleotide molecules, and peptides encoded by these molecules, can be used in the analysis of alloantigen phenotypes, as well as in diagnostic and therapeutic applications relating to human platelet Pl.sup.A polymorphism. In this vein, a method for typing blood cell and platelet membrane glycoproteins entails an analysis of amplified cDNA, encoded by platelet and red blood cell mRNA.
Claims14
An isolated polypeptide between four and fifty amino acids in length having an amino-acid sequence of a fragment of GPIIIa of identical length, wherein said fragment comprises amino acid 33 the GPIIIa.
A method for producing an anti-Pl.sup.A1 or anti-Pl.sup.A2 antibody that distinguishes between the Pl.sup.A2 form of GPIIIa, with a leucine at position 33, and the Pl.sup.A1 form of GPIIIa, with a proline at position 33, comprising the steps of (a) immunizing a mammal with a polypeptide comprising between four and fifty amino acids in length having an amino acid sequence, a fragment of GPIIIa of identical length, wherein said fragment comprises amino acid 33 of the GPIIIa, then (b) removing lymphocytes from said mammal, (c) fusing said lymphocytes with mammalian myeloma cells to form hybridoma cells, (d) culturing said hybridoma cells;
- and thereafter (e) selecting, isolating and cloning hybridoma cells secreting monoclonal antibodies that distinguish between the Pl.sup.A1 and Pl.sup.A2 allelic forms of GPIIIa.
An oligonucleotide that is capable of distinguishing the Pl.sup.A1 allele from the Pl.sup.A2 allele of the GPIIIa gene or GPIIIa cDNA that hybridizes, with a specificity sufficient to distinguish said alleles, to a portion of said gene or said cDNA, respectively, wherein said portion includes a nucleotide corresponding to nucleotide 196 of a GPIIIa cDNA.
Dependencies are inferred from the claim text.