Mammalian inward rectifier potasssium channel cDNAs, host cells expressing them, and screening assays using such cells
AssigneeThe Regents of the University of California
InventorsLily Y. Jan, Yuh Nung Jan, Yoshihiro Kubo, Eithan Reuveny, Paul A. Slesinger
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Abstract
This disclosure relates to two separate and distinct inward rectifier K.sup.+ channel expression products and the the genes which encode each expression product. The IRK1 gene (SEQ ID NO:1) encodes an inward rectifier K.sup.+ channel and the GIRK1 gene (SEQ ID NO:3) encodes a G protein coupled muscarinic K.sup.+ channel. The disclosure relates to the uses of these expression products, particularly in combination with identifying physiological processes mediated by these channels, such as regulation of heartbeat and insulin release and materials modulating or blocking same.
Claims16
An isolated nucleic acid encoding a mammalian inward-rectifying potassium channel protein, having a nucleotide sequence selected from the group consisting of:
- (a) the sequence of a cDNA molecule isolated from a mammalian library, wherein the complementary strand of said cDNA molecule hybridizes under conditions of low stringency with a DNA molecule having a sequence identical to the IRK1 coding sequence shown in SEQ ID NO:
- 3, and
- (b) a sequence degenerate with the sequence of (a).
Dependencies are inferred from the claim text.