Enzymatic reactions and apparatus for carrying them out
AssigneeDegussa AG
InventorsPeter Kuhl, Uwe Eichhorn, Hans-Dieter Jakubke, Karlheinz Drauz, Andreas Bommarius
Life of this patent
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Abstract
A water-supported enzymatic reaction is carried out with the substrate and the enzyme present in the solid phase. Either the water does not form a liquid continuum around substrate and enzyme, or the water acts at least partially out of the gas phase, or the water required for the reaction is added via a solid, or at least a part of the water required for the reaction is transferred by means of solid body contacts or by a combination of these means. The reaction mixture can be mixed by ultrasound or turbulence, e.g. in a fluidized bed, or both.
Claims9
A process involving a water-supported enzymatic reaction for the synthesis of a peptide comprising, forming a solid phase mixture consisting essentially of a substrate, water and a non-immobilized enzyme suitable for the synthesis of the peptide, subjecting the solid phase mixture to conditions suitable for enzymatic activity and intimately mixing the components by the application of ultrasound wherein water is present as a gas or solid and in an amount that is insufficient to form a liquid continuum between substrate and the enzyme but is sufficient for the enzymatic reaction for the synthesis of a peptide to occur.
A process involving a water-supported enzymatic reaction for the synthesis of a peptide comprising, forming a solid phase mixture consisting essentially of a substrate, water and a non-immobilized enzyme suitable for the synthesis of the peptide, subjecting the solid phase mixture to conditions suitable for enzymatic activity and introducing gas under conditions which cause the components of said mixture to intermix wherein water is present as a gas or solid in an amount that is insufficient to form a liquid continuum between substrate and the enzyme but is sufficient for the enzymatic reaction for the synthesis of the peptide to occur.
Dependencies are inferred from the claim text.