Back to results
US 5,670,319 AGranted
Assay for tumor necrosis factor receptor-associated factors
AssigneeGenentech, Inc.
InventorsDavid V. Goeddel, Mike Rothe
Life of this patent
Estimating the term…
Abstract
The invention concerns new tumor necrosis factor receptor associated factors, designated TRAF. The new factors are capable of specific association with the intracellular domain of the type 2 TNF receptor (TNF-R2), and are involved in the mediation of TNF biological activities.
Claims8
An assay for identifying a factor capable of specific binding to the intracellular domain of a native TNF-R2, comprising
- (a) expressing nucleic acid molecules encoding a polypeptide comprising a fusion of an intracellular domain sequence from within amino acids 346-384 to amino acids 423-424 of native human TNF-R2 to the DNA-binding domain of a transcriptional activator, and a fusion of a candidate polypeptide factor to the activation domain of a transcriptional activator, in a single host cell carrying a reporter gene;
- and
- (b) monitoring the binding of said candidate factor to said intracellular domain sequence of TNF-R2 by detecting a signal of the molecule encoded by said reporter gene.
An assay for identifying a factor capable of specific association with the intracellular domain of a native TNF-R2, comprising
- (a) expressing nucleic acid molecules encoding a polypeptide comprising a fusion of an intracellular domain sequence from within amino acids 346-384 to amino acids 423-424 of native human TNF-R2 to the DNA-binding domain of a transcriptional activator, and a fusion of a candidate factor to the activation domain of a transcriptional activator, in a single host cell transfected with nucleic acid encoding a polypeptide factor capable of specific binding to said intracellular domain sequence of TNF-R2, and with nucleic acid of a reporter gene;
- and
- (b) monitoring the association of said candidate factor with said intracellular domain sequence of TNF-R2 or with said polypeptide factor capable of specific binding to said intracellular domain sequence of TNF-R2 by detecting the signal of the polypeptide encoded by said reporter gene.
Dependencies are inferred from the claim text.