Cryoprotective aqueous solutions useful for the preservation of in vitro cultured epithelial sheets
AssigneesDevelopment Biotechnological Processes S.N.C Di Pelliccia Maria Teresa, Consorzio per la Gestione del Centro di Biotecnologia Avanzata
InventorsGiuseppe Geraci, Mario De Rosa, Mose Rossi, Ranieri Cancedda, Michele De Luca, Graziella Pellegrini
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Abstract
A method for the preservation of in vitro expanded epithelial sheets by incubation of the sheets in a cryopreserving solution characterized by lack of volume increase at the liquid-solid phase transition. The cryopreserving solutions comprise: a) polyethyleneglycols having molecular weight between 3 and 5 KD; and b) at least one cross-linking agent selected from polyols, polyamines, mono- or oligosaccharides, and polyethyleneglycols having molecular weight lower than 1 KD.
Claims6
Cryoprotective aqueous solutions for preservation of in vitro cultured epithelial sheets comprising:
- a) from 10 to 20% w/w of polyethyleneglycols having molecular weight from 3 to 5 KD;
- b) from 5 to 25% w/v of at least one cross-linking agent selected from the group consisting of polyols, polyamines, mono- and oligosaccharides, and polyethyleneglycols
- having a molecular weight lower than 1 KD;
- and
- wherein a) and b) are in proportions sufficient to provide a cryoprotective aqueous solution for epithelial sheets.
Dependencies are inferred from the claim text.