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AssigneeThe Regents of the University of Michigan
InventorsStephen G. Emerson, Michael F. Clarke, Bernhard O. Palsson
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Methods, including culture media conditions, which provide for ex vivo human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
An ex vivo cell culture composition comprising human stem cells found in the human hematopoietic system, cultured in a liquid culture medium which is replaced at a rate which is substantially continuous and provides ex vivo human stem cell division, wherein said human stem cells are rendered mitotic ex vivo.
A cellular composition obtained by culturing cellular composition comprising human stem cells found in the human hematopoietic system in a liquid culture medium which is replaced at a rate which is substantially continuous and provides exvivo human stem cell division, while maintaining said culture under physiologically acceptable conditions.
A cell composition obtained by culturing a cellular composition comprising (i) human hematopoietic stem cells or (ii) human bone marrow cells, in a liquid culture medium which is replaced at a rate which is substantially continuous and provides ex vivo human stem cell division therein while maintaining said culture under physiologically acceptable conditions.
In an autologous or an allogeneic human bone marrow transplantation, the improvement comprising using a cell composition obtained by culturing (i) human bone marrow cells or (ii) a cellular composition comprising human hematopoietic stem cell in a liquid culture medium which is replaced at a rate which is substantially continuous and provides ex vivo human stem cell division therein, while maintaining said culture under physiologically acceptable conditions.
A cell composition for use in human blood transfusions obtained by culturing a cellular composition comprising (i) human hematopoietic stem cells or (ii) human hematopoietic cells, in a liquid culture medium which is replaced at a rate which is substantially continuous and provides ex vivo human stem cell division therein, while maintaining said culture under physiologically acceptable conditions.
A human cell culture composition comprising cultured mitotic human stem cells found in the human hematopoietic system and an expanded population of human progenitor cells found in the human hematopoietic system obtained by culturing a cellular composition comprising human stem cells found in the human hematopoietic system in a liquid culture medium which is replaced at a rate which is substantially continuous and provides ex vivo human stem cell division and human progenitor cell expansion therein, while maintaining said culture under physiologically acceptable conditions.
A cellular composition obtained by culturing human progenitor cells found in the human hematopoietic system in a liquid culture medium which is replaced at a rate related to the cell density of the culture to thereby obtain expansion of said human progenitor cellA, said rate being equal to a rate of from 50 to 100% daily replacement for a cell density of from 1.times.10.sup.4 to 1.times.10.sup.7 cells per ml of culture, while maintaining said culture under physiologically acceptable conditions.
A cell culture composition comprising mitotic human stem cells, wherein said composition is obtained by culturing cells derived from human bone marrow in a liquid culture medium which is replaced at a rate which is substantially continuous and provides ex vivo human stem cell division.
An ex vivo cell culture composition in which a substantial number of human stem cells originating from a human hematopoietic system have undergone ex vivo cell division as a result of the conditions in a medium-exchange ex vivo culture.
An ex vivo human hematopoietic or stromal stem cell composition in which a substantial number of human hematopoietic or stromal stem cells have undergone ex vivo cell division as a result of the conditions in a medium-exchange ex vivo culture.
An ex vivo human bone marrow stem cell composition in which a substantial number of human bone marrow stem cells have undergone ex vivo cell division as a result of the conditions in a medium-exchange ex vivo culture.
Dependencies are inferred from the claim text.