Pharmacological preparations comprising human pluripotent hematopoietic colony stimulating factor
AssigneeSloan-Kettering Institute
InventorsKarl Welte, Erich Platzer, Janice L. Gabrilove, Roland Mertelsm, Malcolm A. S. Moore
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Abstract
Highly purified Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a glycoprotein (MW 19,600) constitutively produced by human tumor cells has been highly purified from low serum-containing conditioned medium to apparant homogeneity. Pluripotent CSF supports the growth of human mixed colonies (CFU-GEMM), granulocyte-macrophage colonies (CFU-GM), early erythroid colonies (BFU-E) and induces differentiation of human leukemic cells. The specific activity of the purified pluripotent CSF in the CFU-GM assay is 1.5.times.10.sup.8 U/mg protein.
Claims10
A pharmacological preparation containing isolated and purified human pluripotent colony stimulating factor comprising the following characteristics:
- a) a molecular weight of about 19,600 daltons under reducing and non-reducing conditions as determined by SDS-PAGE;
- b) a molecular weight of about 32,000 daltons as determined by gel filtration;
- c) an isoelectric point of about 5.5;
- d) the ability to induce differentiation of the leukemia cell line WEHI-3B (D.sup.+);
- and
- e) the ability to stimulate growth of both human and mouse bone marrow cells.
Dependencies are inferred from the claim text.