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US 5,670,134 AGranted
Method for evaluating and modifying biological activity
AssigneeGenentech, Inc.
InventorDavid W. Martin, Jr.
Life of this patent
Estimating the term…
Abstract
Biological effects of agents for diagnostic or therapeutic use are evaluated by administration of the agents to transgenic animals which are transformed with heterologous DNA and which are immune tolerant to the expression product of the heterologous DNA. In a further embodiment, preparations that are immunogenic in the transgenic animal model are purified by reverse immunoaffinity chromatography on antibody obtained from responding transgenic animals.
Claims7
A method for detecting the immunogenicity of a potentially immunogenic polypeptide which comprises:
- (a) administering to a transgenic non-human mammal immunologically tolerant to a heterologous polypeptide as the result of the expression of a DNA sequence encoding said heterologous polypeptide, said DNA sequence having been introduced into said mammal or an ancestor of said mammal during an embryonic stage and being transcribed and continuously expressed under the control of a promoter, said potentially immunogenic polypeptide, wherein said potentially immunogenic polypeptide is a variant of said heterologous polypeptide;
- and
- (b) detecting the presence of antibodies specific for said potentially immunogenic polypeptide, the presence of said antibodies being indicative of the immunogenicity of the polypeptide.
Dependencies are inferred from the claim text.