Peptides method for radiolabeling them, and method for detecting inflammation
AssigneeRhomed Incorporated
InventorPaul O. Zamora
Life of this patent
Estimating the term…
Abstract
High affinity chemotactic peptides containing a biological-function domain and a medically useful metal ion-binding domain are labeled with medically useful metal ions for use in diagnosis and treatment of infections, inflammations and related conditions.
Claims22
A peptide comprising a biological-function domain which causes the peptide to accumulate at a target locus and a metal ion-binding domain, wherein the metal ion-binding domain comprises an amino acid sequence selected from the group consisting of Gly-Gly-Cys, Gly-Gly-Pen, Gly-Gly-Gly-Cys and Gly-Gly-Gly-Pen, and D-stereoisomers thereof.
A method for labeling a peptide with a metal ion, comprising the steps of contacting a solution of a peptide sequence containing a biological-function domain and a metal ion-binding domain, wherein the metal ion-binding domain comprises an amino acid sequence selected from the group consisting of Gly-Gly-Cys, Gly-Gly-Pen, Gly-Gly-Gly-Cys and Gly-Gly-Gly-Pen, and D-stereoisomers thereof, with a metal ion, and recovering radiolabeled peptide.
A method for radiolabeling a peptide containing a biological function domain and a metal ion-binding domain, wherein the metal ion-binding domain comprises an amino acid sequence selected from the group consisting of Gly-Gly-Cys, Gly-Gly-Pen, Gly-Gly-Gly-Cys and Gly-Gly-Gly-Pen, and D-stereoisomers thereof, with a radioisotope of technetium or rhenium, comprising the steps of:
- a) contacting a solution of the peptide with stannous ions, wherein the stannous ions are sufficient to reduce the radioisotope added in a subsequent step;
- b) reacting the solution of the peptide sequence and stannous ions with the radioisotope;
- c) recovering the radiolabeled peptide.
A method of detecting at least one of the existence and locus of infection or inflammation in the body of a mammalian subject suspected of suffering from infection or inflammation, the method comprising:
- a) administering to said subject a peptide having an amino acid sequence comprising a biological-function domain which causes the peptide to accumulate at the locus of infection or inflammation and a metal ion-binding domain, and the metal ion-binding domain comprises an amino acid sequence selected from the group consisting of Gly-Gly-Cys, Gly-Gly-Pen, Gly-Gly-Gly-Cys and Gly-Gly-Gly-Pen, and D-stereoisomers thereof, said peptide bearing a detectable metal ion which has been coupled to said metal ion binding domain;
- and
- b) detecting the metal ion bearing peptide, and thereby determining the existence and locus of infection or inflammation.
Dependencies are inferred from the claim text.